Fig. 4.

P10G20 efficiently scavenges free radicals both in vitro and in cell culture. a,b,c) Results showing the scavenging rate of ABTS⁺· (a), DPPH· (b), and hydroxy radical (c) by P10G20 compared with ascorbic acid solutions (VC). d) Scheme showing antioxidant cell model. The oxidative stress was induced by adding tert-butyl hydroperoxide solution (tBOOH). As control, the tBOOH and different weight of P10G20 were added together to show if P10G20 could rescue cells from oxidative stress caused by tBOOH. e,f) Metabolic activity (e) and live/dead cell staining (f) results of NIH3T3 cells when incubated with tBOOH alone and tBOOH with P10G20 of different weight. (Scale bar = 500 μm, n = 6, mean ± SD).