Fig. 7.

P10G20 showed promising therapeutic potential in an in vivo model of IUA. a) Scheme showing that the endometrium was scratched with a curved hook to build the IUA model. P10G20 group received an injection of P10G20. The Saline group was rinsed with saline solution. The healthy uterus was used as the control. b) Pictures showing the process of the experiments, including modelling, saline, and P10G20 treatment, and tissue observation on day 7. Scale bar = 1 cm. c,d) H&E (c) and Masson (d) staining results of the rat uteri in different groups on day 7. The parts inside the black frame are the wounded uterine areas. (Black scale bar = 600 μm, white scale bar = 50 μm. e) The ratio of uterine diameter in the P10G20 and Saline groups to those of the healthy group on day 7. (n = 6, mean ± SD, **p < 0.01) f) The results showing endometrial thickness in different groups calculated by H&E staining on day 7. (n = 6, mean ± SD, ns: not significant) g) The results showing the number of endometrial glands in different groups calculated by H&E staining on day 7. (n = 6, mean ± SD, ns: not significant) h) The results showing the endometrial fibrous tissue area in different groups calculated by Masson staining on day 7. (n = 6, mean ± SD, ns: not significant, ***p < 0.001) Statistical significance was calculated with Student's t-test in e, f, and g, with ANOVA with a Tukey post-hoc test in h.