Table 1.
Proteins with diverse functions are required for crossover interference
| Protein | Species | Function | Interference assay | Reference |
|---|---|---|---|---|
| Synaptonemal complex (SC) | ||||
| Zip1 | S. cerevisiae | Transverse filament of the SC | NPD ratio | Sym and Roeder, 1994 |
| SYP-1 | C. elegans | Cytology | Libuda et al., 2013 | |
| Zyp1 | A. thaliana | Cytology | Capilla-Pérez et al., 2021 | |
| Asy1 | A. thaliana | Cytology | Lambing et al. 2020 | |
| Zep1 | O. sativa | Cytology | Wang et al., 2010 | |
| Sycp1 | M. musculus | Cytology | de Boer et al., 2006 | |
| DPY-28 | C. elegans | Condensin I subunit; chromosomal axis formation | Cytology | Tsai et al., 2008 |
| Regulating CO formation | ||||
| Msh4-Msh5 | S. cerevisiae | Binds Holliday junctions; facilitates CO formation | NPD ratio | Novak et al., 2001; Argueso et al,. 2004; Getz et al., 2008 |
| NPD ratio and CoC | Nishant et al., 2010 | |||
| CoC | Anderson et al., 2015 | |||
| Zip3 | S. cerevisiae | SUMO E3 ligase; required for SC formation and CO formation | CoC | Anderson et al., 2015 |
| Mlh1-Mlh3 | S. cerevisiae | Endonuclease; binds and resolves Holliday junctions | NPD ratio | Argueso et al,. 2003 |
| Slx4 | S. cerevisiae | Endonuclease; resolves Holliday junctions | NPD ratio | Higashide and Shinohara, 2016 |
| Sgs1 | S. cerevisiae | DNA helicase; chromosome synapsis; meiotic joint molecule and CO formation | NPD ratio | Oh et al., 2007 |
| CoC | Anderson et al., 2015 | |||
| Blm | D. melanogaster | CoC | Hatkevich et al., 2017 | |
| Mer3 | S. cerevisiae | DNA helicase; promotes CO formation | NPD ratio | Nakagawa and Ogawa, 1999 |
| Spo16 | S. cerevisiae | Facilitates SC and CO formation | NPD ratio | Shinohara et al., 2008 |
| Spo22 (Zip4) | S. cerevisiae | TPR-like repeat protein; facilitates SC formation and CO formation | NPD ratio | Tsubouchi et al., 2006; Shinohara et al., 2008 |
| Pch2 | S. cerevisiae | Regulating chromosome synapsis and CO formation | NPD ratio | Joshi et al., 2009; Zanders and Alani, 2009 |
| Pch2 | A. thaliana | CoC | Lambing et al., 2015 | |
| RTEL-1 | C. elegans | CO formation | Cytology | Youds et al., 2010 |
| DNA damage check point | ||||
| Tel1 | S. cerevisiae | Protein kinase regulating DNA double-strand-break response | CoC | Anderson et al., 2015 |
| Tel1 | S. pombe | CoC | Fowler et al., 2018 | |
| ATM | M. musculus | Cytology | Barchi et al., 2008 | |
| Topoisomerase II and SUMOylation | ||||
| Top2 | S. cerevisiae | DNA topoisomerase; binds axial cores | Cytology | Zhang et al., 2014b |
| Ndj1 | S. cerevisiae | Regulating meiotic SPB cohesion and telomere clustering | NPD ratio | Chua and Roeder, 1997 |
| Slx5/8 | S. cerevisiae | SUMO-targeted ubiquitin ligase (STUbL) | Cytology | Zhang et al., 2014b |
| Sir2 | S. cerevisiae | Activation of STUbL activity of Slx5/8 | Cytology | Zhang et al., 2014b |