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. 2023 Feb 11;42(14):1072–1087. doi: 10.1038/s41388-023-02619-4

Fig. 3. NEDD4 promotes proliferation of IGF1R-dependent GC cells.

Fig. 3

A Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after Dox-induced silencing of NEDD4. NEDD4-con, scramble shRNA; NEDD4-sh1 and sh2, NEDD4 shRNA constructs 1 and 2. B Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after NEDD4 overexpression. Cell proliferation ability detection of BGC803 and MKN45 cells after NEDD4 knockdown and overexpression by (C, D) CCK-8 assay, (E) plate clone formation assay, (F) soft agar clone formation assay, and (G, H) EdU assay. I Western blot analysis of p-Akt s473 and t-Akt protein levels in BGC803 and MKN45 cells after treatment with Heclin (10 μM) for 48 h. J Cell viability detection of cells treated with Heclin (10 μM) by CCK-8 assay. ***p < 0.001, **p < 0.01, *p < 0.05, ns > 0.05.