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. 2023 Mar 16;24(4):612–624. doi: 10.1038/s41590-023-01448-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a. Force-directed graphs of γδ Vδ2+ T cells are shown. Annotation of phenotypically similar nodes (clusters) are highlighted on force-directed graphs with the same color. Each color corresponds to a phenotypically distinct cluster (10 clusters total). Enriched markers for each cluster are highlighted in color-matched text boxes. b. Force-directed graph colored by tissue source is shown. c. Heatmap showing the differential expression of surface markers used for clustering. Dendrograms show the results of hierarchical clustering used to group similar clusters (columns) and markers with similar cluster distributions (rows). d. Bar plots showing per tissue total cell frequencies of each phenotypic cluster.