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. 2023 Mar 30;14:1775. doi: 10.1038/s41467-023-37327-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Cartoon of the apical complex in the protruded state, highlighting the APR and IMC structures. b A tomographic slice (longitudinal orientation) shows two distinct APR rings (red arrowheads) and the AAD ring (purple arrowhead), which is located between the APR and IMC. c A cross-sectional tomographic slice shows the IMC near the apical edge with “spacer” densities (cyan arrowheads) between the two membranes. d, e Tomographic slices through the protruded conoid complex from two parasites. Measurements mark the minimum distances between the IMC and the conoid fibers on each side of the conoid. f, g Tomographic slice (f: original; f’: pseudo-colored) and 3D-segmented isosurface rendering (g) of the same region at the base of a protruded conoid show filamentous actin-like densities (magenta) connecting between the conoid (orange) and the APRs (red). Scale bars: 100 nm (in b, d, e); 50 nm (in c, f).