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. 2023 Mar 30;14:1775. doi: 10.1038/s41467-023-37327-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Cartoon of the apical complex highlighting the location of the PCRs. b–d Tomographic slices (b and c), and isosurface rendering of the averaged PCR repeats (d) viewed in the tangential (b) and longitudinal (c and d) orientations, showing different components of the PCR including the apical P1 (highlighted by blue arrowheads), P2a (cyan), P2b (green), P3 (yellow), and the linker (orange) between P2b and P3. The white line in (c) indicates the location of the slice in (b). e, f Cross-sectional slices from a raw tomogram (e) and the averaged PCR repeats (f) show that the round PCR P2 ring is composed of an outer and an inner ring, P2a and P2b, respectively. The yellow square in (e) indicates the orientation and location of the subtomogram average displayed in (f). g, h Isosurface renderings show the complete PCRs by assembling the averaged repeats back to the full tomogram. Scale bars, 20 nm (in b, c, f); 50 nm (in e).