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. 2023 Mar 30;14:1771. doi: 10.1038/s41467-023-36872-8

Fig. 2. A minimal protein composed of the single LNS2-domain of Nrxn3α attached to its C-terminal stalk sequence, transmembrane region and cytoplasmic sequence fully rescues inhibitory synaptic transmission in cultured Nrxn3-deficient OB neurons.

Fig. 2

All experiments were performed in dissociated OB cultures obtained from newborn Nrxn3 cKO mice that were infected with lentiviruses expressing ΔCre (control) or Cre without or with the indicated rescue proteins. a Schematic of Nrxn3α rescue constructs with domain deletions. b, c Nrxn3α lacking LNS5 and LNS6 domains fully rescues impaired inhibitory synaptic transmission in Nrxn3-deficient OB neurons, whereas Nrxn3α lacking LNS1-3 domains does not (b, sample traces; c, summary graphs of IPSC amplitudes). d Schematic of minimal Nrxn3α rescue constructs. e, f A minimal Nrxn3α protein containing only LNS2 without an SS2 insert linked to the C-terminal Nrxn3α sequences, rescues impaired inhibitory synaptic transmission in Nrxn3-deficient OB neurons (e, sample traces; f, summary graphs of IPSC amplitudes). g, h The minimal Nrxn3α protein containing only LNS2 is unable to rescue inhibitory synaptic transmission in Nrxn3-deficient OB neurons if SS2 contains an insert (g, sample traces; h, summary graphs of IPSC amplitudes). Numerical data are means ± SEM; n’s (cells/experiments) are indicated above the sample traces and apply to all graphs in an experimental series. Statistical analyzes were performed with a one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test, with *p < 0.05, **p < 0.01, and ***p < 0.001. Source data and statistical results for all experiments are provided within the Source Data file.