Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 30;13:5219. doi: 10.1038/s41598-023-31554-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The study of mouse vocalizations during natural behavior requires attribution of individual vocalizations to individual mice. (A) A pair of female and male mice interacted freely on an elevated platform. Their spatial location, behavior and vocal production was monitored with a high-speed camera (placed directly above) and 4 ultrasonic microphones surrounding the platform. The whole setup was situated inside a sound-proof, ultrasonically anechoic box which was uniformly illuminated using a planar array of LEDs. (B) Animals were easily distinguishable against the white, anechoic platform. (C) Vocalizations occurred frequently during most experiments, in particular during social interaction of the animals (Frame in (B) at time = 217.1 s). (D) In the present paradigm, the majority of vocalizations were emitted when the animals were in rather close proximity (black: 4 microphone setup, gray: 3 microphone setup), typically below 10 cm snout-to-snout distance, shown on the abscissa.