Progranulin expression increased in the chloroquine and trehalose
treatment groups after tMCAO. (a) Western blot and the quantification of
PGRN in sham group and saline, Chq and Treh group at 3 days after tMCAO,
n = 4–5 per group. (b) Quantitative of RT-PCR analysis for the
expression of PGRN in sham group and saline, Chq and Treh group at 3
days after tMCAO. n = 6–9 per group. c. Representative Confocal imaging
(left) showed the co-localization of PGRN (green) with neurons (MAP2,
red) and microglia (CD11b/P2RY12, red) in saline, Chq and Treh group at
3 days after tMCAO, Scale bar = 50 μm. Proportion of cells expressing
PGRN in microglia and neuron (right), n = 3 per group. Data are
presented as mean ± SD. *p < 0.05,
**p < 0.01, ***p<0.001.