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. 2023 Mar 17;14:1112834. doi: 10.3389/fendo.2023.1112834

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Copyright © 2023 Dumont, Lopez Maestre, Chalmel, Huber, Rives-Feraille, Moutard, Bateux, Rondanino and Rives

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell gene expression during the first spermatogenic wave. Known Sertoli (Vim, Gata4, Ar, Amh, Sox9) (A_1-3), Leydig (Cyp17a1, Cyp11a1, Hsd3b1, Ccn5) (B_1-3), peritubular/blood vessel (Bgn, Dcn, Acta2) (C_1-3), and germ cell (Ddx4)/elongated spermatids (Prm1, Prm2) (D_1-3) markers expressed in the testis or testicular explants during the first wave of spermatogenesis for in vivo (A₁, B₁, C₁, D₁), in vitro (A₂, B₂, C₂, D₂) and fresh vs. frozen (A₃, B₃, C₃, D₃) samples. Horizontal black lines indicate the mean expression values in each group. CSF, controlled slow freezing; dpp, days postpartum.