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. 2023 Mar 2;26(4):106314. doi: 10.1016/j.isci.2023.106314

Figure 6.

Figure 6

A salicylic acid derivative promotes mitochondrial activity and keratinocyte differentiation

(A) Structural formula of C8-SA (C8/capryloyl-salicylic acid).

(B) Impact of C8-SA (10 μM) on the proliferation of normal human epidermal keratinocytes (NHEK). Data are shown as mean of NHEK derived from 3 different donors that were tested independently. Lines indicate independent pairs. ∗p < 0.05, paired Student’s t test.

(C) PPARGC1A, PPARGC1B, IVL, and TGM1 gene expression in NHEK treated with siCTL or PGC-1 DKD for 3 days and with C8-SA (10 μM) or DMSO (control) for the last 48 h. Data are shown as mean +SEM and fold change normalized to siCTL and DMSO conditions of 3 experiments. ∗p < 0.05, two-way ANOVA, Tukey post-test.

(D) OCR of normal human epidermal keratinocytes (NHEK) treated with DMSO or C8-SA (15 μM) for 3 days. Data on graph are shown as mean +SEM from 4 independent NHEK cultures, ∗p < 0.05, paired Student’s t test. Lower inset shows the average proportion of total respiration dedicated to ATP synthesis (coupled respiration) +/− SEM.