REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-GAPDH	Proteintech Group	RRID: AB_2107436
Rabbit polyclonal anti-HSPA8	ABclonal	Cat#A14001; RRID: AB_2313773
Rabbit monoclonal anti-HSP90 beta	Abcom	Cat#ab203085; RRID: AB2313773
Mouse monoclonal anti-PRRSV N	LVDU	Cat#LD062; RRID: AB2313773
Goat anti-Mouse HRP-conjugated Antibody	Bioss	Cat#bs-0293G; RRID: AB2313773
Goat anti-Rabbit HRP-conjugated Antibody	Bioss	Cat#bs-0295G; RRID: AB2313773
Bacterial and virus strains
Porcine reproductive and respiratory syndrome virus (PRRSV)	Isolated from one suspected PRRSV infected piglet by our lab.31	N/A
Chemicals, peptides, and recombinant proteins
Matrine	National Institutes for Food and Drug Control	Cat#110805-201709
Critical commercial assays
Trizol	TaKaRa	Cat#SD1412
Prime Script ™ RT reagent Kit with gDNA Eraser	TaKaRa	Cat#RR047A
2×SYBR Green Low ROX qPCR Master Mix	Bimake	Cat#B21702
RIPA buffer	Solarbio	Cat#R0010
Phosphatase Inhibitor	Bimake	Cat#B15001
BCA protein concentration assay kit	Beyotime	Cat#P0009
4×LDS Sample Buffer	Invitrogen	Cat#NP0008
SDS-PAGE gel preparation kit	Boster	Cat#AR0138
uper-sensitive ECL ready-to-use substance kit	Boster	Cat#AR1173
Deposited data
RCSB PDB	Burley et al.32	https://www.rcsb.org
Uniprot	Bateman et al.33	https://www.uniprot.org
Comparative Toxicogenomics Database	Davis et al.13	https://www.ctdbase.org
PharmMapper	Wang et al.12	https://www.lilab-ecust.cn/pharmmapper/
PubChem	Kim et al.34	https://pubchem.ncbi.nlm.nih.gov
STRING	Szklarczyk et al.35	https://cn.string-db.org
DAVID	Sherman et al.36	https://david.ncifcrf.gov
Experimental models: Cell lines
Monkey Embryonic Kidney Epithelial Cells	China Institute of Veterinary Drug Control	N/A
Oligonucleotides
Primer: GAPDH Forward	This paper	5'GTCAGTGGTGGACCTGACCT-3'
Primer: GAPDH Reverse	This paper	5'TGCTGTAGCCAAATTCGTTG-3'
Primer: HSPA8 Forward	This paper	5'CCCCATCATCACCAAGCTGT-3'
Primer: HSPA8 Reverse	This paper	5'CTCCACCACCAGGAAATCCC-3'
Primer: HSP90AB1 Forward	This paper	5'CATCCCCAACCCTCAGGAAC-3'
Primer: HSP90AB1 Reverse	This paper	5'CAGCAGAAGACTCCCAAGCA-3'
Primer: PRRSV N Forward	This paper	5'AGAAGCCCCATTTCCCTCTA-3'
Primer: PRRSV N Reverse	This paper	5'CGGATCAGACGCACAGTATG-3'
Software and algorithms
Cytoscape Version: 3.7.2	Shannon et al.37	https://cytoscape.org
AutoDockTools Version: 1.5.6	Österberg et al.38	https://autodock.scripps.edu
PyMol Version: 2.5.2	Lill et al.39	https://pymol.org/2/