Table 1.
PCR conditions of the studied polymorphisms
| SNP | Primer sequence | Reaction conditions | product size (bp) | Restriction Enzyme | Fragment Length |
|---|---|---|---|---|---|
|
FPN1 -8CG |
Forward : 5’CCAGTTCCTTGCACTCCTG-3’ Reverse : 5’CATCCTCTCTGGCGGTTG-3’ |
→→5’94oC 30(30’’94oC + 30’’60 oC+ 60’’72oC) 5’72oC | 129 | BstU I |
CC;129 GG; 85, 44 CG;129, 85, 44 |
| TMPRSS6 rs855791 |
Forward : 5’TAGAGAACAGGGGCTCCAGG 3’ Reverse : 5’ATGTGGGCAGCATCCTTTC 3’ |
→→5’94oC 30(45’’95oC + 45’’64oC + 45’’72oC) 5’72oC | 249 | Stu I |
CC;249 TT;125 TC; 249, 125 |
| HJV I222N |
Forward : 5’TAGTCCTGCATCTCTACTTGG 3’ Reverse : 5’AGCTGCCACGGTAAAGTTGG3’ |
→→5’94oC 30(30’’94oC + 30’’60oC + 30’’72oC) 5’72oC | 581 | Bcc I |
TT; 233,159,120,69 AA; 581 TA; 581,233,159,120,69 |
| HJV G320V |
Forward : 5’TAGTCCTGCATCTCTACTTGG 3’ Reverse : 5’AGCTGCCACGGTAAAGTTGG3’ |
→→5’94oC 30(30’’94oC + 30’’60oC + 30’’72oC) 5’72oC | 581 | Ban I |
GG; 409,172 TT; 581 TG; 581,409,172 |