Figure 1.

Tubulin proteins are highly conserved between human and Plasmodium and the effects of polyclonal anti-tubulin antibodies on oocyst development. (A) Sequence alignment of human α-tubulin (HA) and P. falciparum α-tubulin-1 (PfA). Clustal X Colour Scheme was used to show amino acids. (B) Sequence alignment of human β-tubulin (Hβ) and P. falciparum β-tubulin-1 (Pfβ). Clustal X Colour Scheme was used to show amino acids. (C) Standard membrane feeding assays were used to measure Intensity and prevalence of oocysts in the presence of purified rabbit anti-human α-tubulin polyclonal Ab (labeled with α, 0.01 mg/ml ) and purified polyclonal Ab against human β-tubulin (labeled with β, 0.01 mg/ml). A non-related purified rabbit polyclonal Ab (anti-V5, labeled with control, 0.01 mg/ml) was used as the negative control. Mann-Whitney test was used to calculate P-value. N, number of mosquitoes.