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P. falciparum α-tubulin-1 interacts with An. gambiae FREP1. (A) ELISA showed that P. falciparum α-tubulin-1 bound to An. gambiae FREP1. A bar exhibits the actual value of A405 from one replicate, and there are three replicates per treatment. (B) P. falciparum α-tubulin-1 pulled down FREP1 from mosquito midgut lysate, which was detected by a western blotting assay. .
