Fig. 2.

Effects of lyophilization on solid-state ¹³C NMR spectra of EPS. 150 MHz ¹³C NMR spectra of C. neoformans EPS samples obtained with 15 kHz magic-angle spinning (MAS), comparing samples that were native (partially dehydrated) (left) and lyophilized (partially rehydrated) (right). DPMAS experiments with short (2 s) delays between successive cycles of signal acquisition, favoring carbon moieties that tumble rapidly in many directions. Sharp resonances at 40 and 170 ppm are attributed to glycine in the culture media. (No EPS signals are observed in CPMAS experiments that favor rigid carbon moieties with nearby hydrogens.)