Fig. 4.

Lyophilization of C. neoformans EPS alters biological functions. Native EPS and lyophilized and resuspended samples were assayed for anti-GXM mAb binding by capture ELISA. Binding curves (A) of serially diluted mAb 18B7 as a function of EPS concentration in capture ELISA. Native EPS generally binds more strongly to the 2D10/18B7 capture/detection mAb pair than lyophilized EPS at a given mAb/antigen concentration. Statistical analysis (B) of binding in a capture ELISA double-array assay varying both EPS and mAb concentration shows that native EPS is statistically significantly better bound by anti-GXM mAbs than lyophilized EPS. **** p-value < 0.0001.