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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Biomaterials. 2022 Apr 28;285:121536. doi: 10.1016/j.biomaterials.2022.121536

Fig. 4. Endocytosis of Cy5-nanostars in HEK293Tcells.

Fig. 4.

The uptake and intracellular trafficking of PFPA-Cy5 nanostars in HEK293T cells was examined by immunofluorescence and confocal microscopy. Antibodies to early endosomal antigen 1 (EEA1) and Rab7a were used to detect early and late endosomes, respectively. A. Localization of PFPA-Cy5 nanostars and early endosomal antigen 1 at 30, 60 and 120 min. B. Manders’ overlap coefficient for PFPA-Cy5 nanostars and early endosomal antigen 1. C. Localization of PFPA-Cy5 nanostars and Rab7a at 30, 60 and 120 min. D. Manders’ overlap coefficient for PFPA-Cy5 nanostars and Rab7a. N = 6 independent experiments. *P < 0.05, **P < 0.01. 1-way ANOVA, Duns’s multiple comparison test. Scale bar, 10 μm.