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Endothelial cell tube formation assay. (A, D) Vascular network formation and expression of (B, E) CD31 of HUVECs on Fn-cECM (A, B) and cECM (D, E) hydrogels. (C, F) Expression of vWF for HUVECs seeded on Fn-cECM (C) and cECM (F) hydrogels. Scale bar: 200 μm in A, D and 100 μm in B, C, E, and F. (G) Quantification of angiogenesis parameters using ImageJ software. n = 7; *p < 0.05, ****p < 0.0001.
