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. Author manuscript; available in PMC: 2023 Mar 31.
Published in final edited form as: Anal Chem. 2022 Sep 6;94(37):12604–12613. doi: 10.1021/acs.analchem.2c01034

Figure 2.

Figure 2.

Ion images produced from an expanded view of the MALDI-MSI analysis of a tissue section from a cortical nephrectomy block. The tissue was imaged at 20 μm and ion images were obtained by averaging 25 ppm windows for an acetylated and dimethylated histone H4 proteoform at m/z 11,306.3939 (−1.12 ppm) and a diacetylated histone H4 proteoform at m/z 11,320.3661 (−1.74 ppm). Images are all scaled to 200 μm each with separate relative ion abundance. The corresponding microscopic image from the PAS staining post MALDI-MSI is shown at the bottom to highlight atrophic tubular regions with no significant enhancement in signal intensity (orange) and areas of tubular atrophy with the enhancement of the signal (white). Glomeruli are highlighted in yellow.