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. 2023 Apr 3;133(7):e166031. doi: 10.1172/JCI166031

Figure 1. STING signaling–activated DCs exhibit enhanced glycolysis.

Figure 1

(A) Principal components analysis (PCA) of central carbon metabolome of bone marrow–derived DCs (BMDCs) stimulated with 2 μg/mL 2′3′-cGAMP (cGAMP) for 4 hours (n = 4). Each symbol represents data from an individual mouse. NT, nontreated; ST, cGAMP stimulated. (B and C) Heatmap analysis (B) and graph presentation (C) of differential metabolites in NT and ST groups from A. (D and E) Extracellular acidification rate (ECAR; n = 6; D) and oxygen consumption rate (OCR; n = 5; E) of BMDCs stimulated with 2 μg/mL cGAMP for 4 hours under basal (Bas) or maximum (Max) conditions. (F and G) ECAR (n = 5; F) and OCR (n = 6; G) of BMDCs stimulated with 40 μg/mL tumor DNA (Tu-DNA) for 4 hours under Bas or Max conditions. (H) Gene set enrichment analysis of the hallmark glycolysis pathway in the freshly isolated tumor-infiltrating DCs (Tu-DC) compared with that of splenic DCs (Spl-DC). DCs were isolated from MC38 tumor-bearing WT mice on day 14 after tumor injection. (I and J) ECAR (n = 5; I) and OCR (n = 3; J) of splenic and tumor-infiltrating DCs isolated from MC38 tumor-bearing WT mice on day 14 after tumor injection. Representative data are shown from 3 independent experiments in D–G, I, and J. Data are shown as the mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test; *P < 0.05; **P < 0.01.