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. 2023 Apr 3;133(7):e166031. doi: 10.1172/JCI166031

Figure 3. Glycolysis drives STING signaling in DCs.

Figure 3

(A) ECAR of WT and Ldha/b-DKO BMDCs stimulated with 2 μg/mL cGAMP for 4 hours under Bas or Max conditions (n = 5). (B) Relative mRNA expression level of WT and Ldha/b-DKO BMDCs stimulated with 2 μg/mL cGAMP for 3 hours was determined by qRT-PCR. (C) IFN-β protein level of WT and Ldha/b-DKO BMDCs stimulated with 2 μg/mL cGAMP for 8 hours was determined by ELISA. (D) Immunoblot analysis of indicated proteins in whole-cell lysates of BMDCs stimulated with 2 μg/mL cGAMP for 4 hours. (E) ECAR of WT and Ldha/b-DKO BMDCs stimulated with 40 μg/mL Tu-DNA for 4 hours under Bas or Max conditions (n = 5). (F) Relative mRNA expression level of WT and Ldha/b-DKO BMDCs stimulated with 40 μg/mL Tu-DNA for 3 hours was determined by qRT-PCR. (G) IFN-β protein level of WT and Ldha/b-DKO BMDCs stimulated with 40 μg/mL Tu-DNA for 8 hours was determined by ELISA. (H) Immunoblot analysis of indicated proteins in whole-cell lysates of BMDCs stimulated with 40 μg/mL Tu-DNA for 4 hours. The numbers indicate the relative densities of indicated protein bands normalized to β-actin. Representative data are shown from 3 independent experiments. Data are shown as the mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test; **P < 0.01.