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. 2023 Apr 3;133(7):e166031. doi: 10.1172/JCI166031

Figure 7. STING signaling promotes glycolysis and enables a positive feedback circuitry.

Figure 7

(A) Immunoblot analysis of indicated proteins in whole-cell lysates of BMDCs stimulated with 2 μg/mL cGAMP for 4 hours. (B) Functional enrichment analysis of KEGG pathways in BMDCs stimulated with 2 μg/mL cGAMP for 4 hours. (C) Immunoprecipitation assays using indicated antibodies in BMDCs stimulated with 2 μg/mL cGAMP in the presence or absence of TEPP-46 (100 μM) for 4 hours. (D) Immunoblot analysis of indicated proteins in whole-cell lysates of splenic DCs (Spl-DC) and tumor-infiltrating DCs (Tu-DC) isolated from MC38 tumor-bearing WT mice on day 14 after tumor inoculation. (E–G) ECAR (E), immunoblot analysis (F), and qRT-PCR analysis (G) of BMDCs stimulated with 2 μg/mL cGAMP in the presence or absence of TEPP-46 (100 μM) for 4 hours. (H–J) Immunoblot analysis (H), qRT-PCR analysis (I), and ECAR (J) of tumor-infiltrating DCs from WT and Tmem173–/– (KO) mice i.p. injected with 50 mg/kg TEPP-46 on days 3, 5, and 7 after MC38 tumor cell inoculation. Ctrl, without TEPP-46 injection. (K and L) Immunoblot analysis (K) and qRT-PCR analysis (L) of human NSCLC DCs treated with TEPP-46 (100 μM) for 8 hours. The numbers indicate the relative densities of indicated protein bands normalized to β-actin. Data are representative of 3 independent experiments (A and C–L). Data are shown as the mean ± SEM. Statistical analysis was performed using 1-way ANOVA (I and J) and 2-tailed Student’s t test (E, G, and L); *P < 0.05; **P < 0.01.