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. 2023 Apr 3;133(7):e164413. doi: 10.1172/JCI164413

Figure 4. NF-κB and STAT3 signaling mediate survival of resistant Kras+/–;Trp53–/– clones.

Figure 4

(A) Western blot analysis of phospho-p65 (p-p65), p65, p-STAT3, and STAT3 expression in parental Kras+/G12Vlox;Trp53–/– cell lines (P3 to P6) and resistant Kras+/–;Trp53–/– clones (R1 to R9). GAPDH served as loading control. (B) Colony formation assays on 10 cm cell culture dishes of parental Kras+/G12Vlox;Trp53–/– cell lines and resistant Kras+/–;Trp53–/– clones expressing either nontargeting shRNA or shRNAs against p65 and/or STAT3. (C) Quantification of the number of colonies present in the experiment described in B. Data are represented as mean ± SEM. P values were calculated using 2-way ANOVA. **P < 0.01; ***P < 0.001. (D) Viability of parental Kras+/G12Vlox;Trp53–/– cell lines (n = 4) and resistant Kras+/–;Trp53–/– clones (n = 6) treated with BAY 11-7082 (10 μM) and Stattic (5 μM) either individually or in combination (Combo). Data are represented as mean ± SEM. P values were calculated using 2-way ANOVA. ***P < 0.001.