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. 2023 Apr 3;133(7):e164185. doi: 10.1172/JCI164185

Figure 1. Structure of the Agrp gene and mRNA variants.

Figure 1

(A) The exon-intron structure is shown schematically for the Agrp gene and its mRNA variants. Coding exons are shaded in gray. Each of the first exons (1A, 1B, and 1C) is presented in a different shade. The size of each intron is indicated below the intron. Each mRNA variant is presented with its included exons. Note that transcript C has an alternatively spliced exon (1C′). The number of spliced transcripts found from RNA-Seq studies, as reported in the NCBI Genome Data Viewer, is presented in italic text at the intron positions. Unfortunately, there are no data regarding the number of reads corresponding to intron-spanning reads that include exon A. (B) Results of mRNA variant–specific amplification from hypothalamic block RNA isolated from an adult male mouse. Total AGRP represents amplification for all AGRP mRNA variants, and each subsequent lane represents amplification for mRNA variants A–C.