Figure 11. Deletion of astrocytic OGT in the mPFC protects neuronal calcium activity from stress.

(A) Schematic of the AAV vectors engineered to express a calcium sensor (GCaMp6s) under a syn promoter. (B and C) Representative images of the injection sites in the mPFC and paradigms of the FIT. Scale bars: 500 μm (right, magnified view of the left image); 50 μm (left). (D) Representative heatmaps of z score Ca²⁺ signal changes over all trials from single mice. (E and F) Time course of average GCaMp6s transient z scores event-locked to SI (E) and the quantification of the average peak z score during SI (F). n = 10 (WT); n = 7 (cKO) mice. Data are represented as mean ± SEM. Two-way ANOVA with Bonferroni’s multiple comparisons test (F). **P < 0.01. See Supplemental Data Set 1 for statistical details.