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. 2023 Apr 3;133(7):e160016. doi: 10.1172/JCI160016

Figure 4. Astrocyte-specific deletion of OGT in the mPFC results in antidepressant effects.

Figure 4

(A) Schematic of the AAV vectors engineered to specifically knock down astrocytic OGT in the mPFC and behavioral test paradigms. (B) Representative images of injection sites in the mPFC of OGTfl/Y mice. Scale bars: 500 μm (right, magnified view of the left image): 20 μm (left). (C and D) Western blot images (C) and quantification (D) of OGT in the mPFC of OGTfl/Y mice. n = 6 (Ctrl); n = 6 (iCre) mice. (E) Immobility time of mice with selective OGT knockdown in the FST. n = 11 (Ctrl); n = 14 (iCre) mice. (F) SI time in the absence or presence of social targets before and after CSDS. n = 14 (Ctrl); n = 13 (iCre) mice. (G) Representative heatmaps of selective OGT-knockdown mice in the presence of social targets before CSDS (left) and after CSDS (right). Data are represented as mean ± SEM. Two-sided unpaired t test (D and E); 2-way ANOVA with Bonferroni’s multiple-comparisons test (F). *P < 0.05; **P < 0.01. See Supplemental Data Set 1 for statistical details.