Fig 6. Enhancing antimicrobial potency while exhibiting no toxicity to mammalian cells.

A-B: Concentration-dependent toxicity by CellTiter Blue assay on HEK 293T cells for P2 and derivatives with improved antimicrobial potency. A: Derivatives with increased aggregation propensity or compatibility to the aggregate core structure. B: Derivatives of linker modification or cysteine addition. C: Derivatives of altered number of arginine residues. Error bars represent SEM. Two-tailed Student t-test was performed for calculating statistical significance between the mean of P2 variant and P2 at the corresponding concentration (n = at least 6). Asterisks indicating the level of the p-value centred over the error bar mean: *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.