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. Author manuscript; available in PMC: 2023 Mar 31.
Published in final edited form as: Front Ecol Evol. 2022 Feb 15;10:769433. doi: 10.3389/fevo.2022.769433

FIGURE 7 ∣.

FIGURE 7 ∣

Expression of organizer genes Pf-Gsc and Pf-FoxA. (A–D) Whole mount in situ hybridization of Pf-Gsc at (A) 3 days post-amputation (dpa), (B) 4 dpa, (C) 5 dpa, and (D) 7 dpa of regeneration, respectively. Pf-Gsc marks the ventral base of the blastema, which become the base of the proboscis in all samples stained. (E–I) Whole mount in situ hybridization of Pf-FoxA at 3–8 dpa of regeneration. (E) Pf-FoxA is expressed at the ventral margin of the blastema, lateral to the Pf-Gsc signal, which becomes the edge of collar at 3 dpa. (F) At 4 dpa, dorsal view (G) at 4 dpa, (H) at 7 dpa, (I) at 8 dpa, ventral view. Ventral is facing the viewer in all figures except (F,G) in which the proboscis is facing the viewer. p, nascent proboscis; c, nascent collar. Dorsal is up in all panels except panel (G). Scale bars = 0.25 mm.