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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: Transl Stroke Res. 2022 Oct 1;14(5):766–775. doi: 10.1007/s12975-022-01093-6

Figure 1. Creation and confirmation of mouse GPR39 knockout model.

Figure 1.

A. Schematic of mouse GPR39 gene locus targeting strategy. Guide RNAs were designed to cleave at sites flanking exon 1 of Gpr39 resulting in a 1322 base pair deletion. Exon 2 was left intact. B. RT-PCR and C. Western blot of GPR39 (top row) and beta-Actin (bottom row) confirming presence of GPR39 mRNA (B) and protein (C) in brain, heart and kidneys from wild type mice (left) but absent in GPR39 knockout mice (right).