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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: J Immunol. 2022 Dec 1;209(11):2149–2159. doi: 10.4049/jimmunol.2200324

FIGURE 3. CD8 T cells from TriVax-immunized CoH mice display reduced differentiation and function after restimulation.

FIGURE 3.

A. Experimental design – SPF and CoH B6 mice were immunized with B8R-TriVax. On days 7 and 30 after immunization, spleens were isolated and processed for flow cytometry to determine the (B) frequency and number of B8R-specific CD8 T cells. C-D. Day 7 post-TriVax samples were examined for KLRG1 and CD127 expression to define MPEC (KLRG1−CD127+) and SLEC (KLRG1+CD127−) frequency. E-G. Day 7 and 30 post-TriVax samples were examined for KLRG1 and CD62L expression to define the frequency and number of KLRG1−CD62L− (TEM), KLRG1−CD62L+ (TCM), and KLRG1+CD62L− (LLEC) subsets. Data are representative of at 3 technical replicates consisting of 3–4 mice/group/timepoint. * p ≤ 0.05, ** p ≤ 0.01. H. Experimental design – SPF and CoH B6 mice were immunized with B8R-TriVax. After 7 and 30 days, spleens were collected and processed into a single cell suspension for in vitro restimulation with B8R peptide to induce effector cytokine (IFNγ and TNFα) production. I. The frequency of IFNγ+ and IFNγ+TNFα+ cells among the CD44hi CD8 T cells within the culture was determined by flow cytometry. Data are representative of 2 technical replicates consisting of 3–4 mice/group/timepoint. * p ≤ 0.05