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. 2023 Mar 13;8(4):629–639. doi: 10.1038/s41564-023-01339-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — Following fractionation of organoid cultures, all fractions were visually examined on a tissue culture microscope to assess collection of target populations. (a) Visual examination of the Organoid fraction at 40x magnification confirms that organoids are collected with gravity pellet (1 x g). (b) Region highlighted in A. Single cells were also present at low abundance in the organoid fraction (arrows indicate examples), potentially a result of ongoing cell extrusion from organoids during fractionation (arrowheads).