Extended Data Fig. 3. Infected cells accumulate in organoids after 7 h infection and diminish after 9 h.
(a) Infected organoids were fixed at timepoints over the first cycle of infection and the number of infected and uninfected cells were enumerated using fluorescence microscopy. Each dot represents the proportion of infected cells within an individual organoid; triangles represent values averaged across all organoids for each timepoint. One experiment with N = 10 organoids quantified per timepoint. A peak in the proportion of infected cells within organoids emerged at 7 hpi and was reduced after 9 hpi. Raw cell numbers represented in this graph are displayed in Supplementary Table 2. (b–d) Representative infected cells from each timepoint. Extruding infected cells contain similarly abundant vRNA signal regardless of timepoint visualized. Scale bars equal 10 μm.