Figure 3.

Knockdown of KIF2C inhibits NPC cell growth. (A) RNAi-mediated KIF2C gene silencing. Human NPC cells (HNE-1 and CNE-1) were infected with lentivirus particles expressing negative control shRNA (NCsh) and shRNA against KIF2C (KIF2Csh). Forty-eight hours after infection, total RNA and whole cell lysates were prepared and subjected to RT-PCR and immunoblotting, respectively. (B) KIF2C knockdown causes changes in cell morphology. Cell morphology was monitored under phase-contrasted microscope at the indicated times after lentivirus infection (× 100). (C, D) KIF2C knockdown inhibits HNE-1 and CNE-1 cell growth, respectively. Cell growth was continuously monitored by JULI Stage Real-time Cell History Recorder, and cell viability was examined by trypan blue exclusion assay at the final time point.