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. 2023 Mar 31;14(3):229. doi: 10.1038/s41419-023-05756-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — RNA-sequencing was performed using HCT116 cells stably overexpressing Nodal and control cells. The experiment was repeated thrice for each cell type. A Volcano map demonstrating the distribution of DEGs in HCT116 cells in the vector/oe-Nodal group. B Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis showed the signalling pathways that were most enriched by significantly differentially expressed genes. C Heatmap demonstrating the relative protein abundance. D The mRNA expression of SCD1 in 75 CRC tissues and their adjacent normal tissues was quantified via a qRT-PCR. E Correlation between SCD1 expression and the metastasis and node stages in the collected CRC samples. F Representative images of IHC staining for SCD1 in CRC tissues and their adjacent normal tissues (magnification, × 200). G Western blotting was performed to examine the protein expression of SCD1 in six pairs of CRC tissues. H Pearson correlation analysis of the correlation between SCD1 and Nodal expression in the collected CRC samples. The relative expression level was calculated by using the 2^-△△CT method. I Dual IF assay showed the expression and distribution of the SCD1 (green) and Nodal (red) in two CRC tissues (magnification, 200×). (^∗P < 0.05 and ^∗∗∗P < 0.001).