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. 2023 Mar 31;14(3):229. doi: 10.1038/s41419-023-05756-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Schematic diagram for the preparation of BSA-NP/si-Nodal nanocomplexes. B Agarose gel retardation assay was performed to evaluate the encapsulation efficiency of BSA-NPs. C Transmission electron microscopic images of BSA-NP/si-Nodal nanocomplexes. D, E The hydrodynamic particle size and zeta potential distribution of BSA-NP/si-Nodal complexes were measured via dynamic light scattering. F Western blot analysis of Nodal, SCD1, Smad2/3, p-Smad2/3, E-cadherin, N-cadherin and snail expressions in cells with different treatments. G Schematic diagram of in vivo experiments in tumour-bearing mice models. H–J Tumour images, tumour volume and tumour weight in mice. K IHC analysis of the Ki67 and Nodal expressions. L Fluorescence images and fluorescence intensity analysis of nude mice with lung metastasis (^∗∗P < 0.01 and ^∗∗∗P < 0.001).