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. 2023 Mar 31;14:1810. doi: 10.1038/s41467-023-37499-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a PC-3 and U2OS cells infected with lentivirus expressing indicated shRNAs were treated with IR for 1 h and harvested for WB analysis. b Ubiquitination assay using Ni-NTA pull-down in 293T cells transfected with His-K29-single ubiquitin together with other indicated plasmids and treated with IR (5 Gy) for 1 h. c Ubiquitination assay using Ni-NTA pull-down in 293T control and TRABID knockdown cells transfected with indicated plasmids. Cells were harvested at different time points after IR. d–f U2OS cells infected with lentivirus expressing indicated shRNA were treated with IR followed by IFC of 53BP1 at the indicated time points after IR (d). Scale bar, 10 µm. The average 53BP1 foci number (e) and foci density (f) in each cell were quantified. Data were presented as means ± SD of more than 300 cells from three biological replicates. Two-tailed unpaired Student’s t-test. n.s., not significant. P values based on the order of appearance: e (0.154, 0.08, 0.054, 3E-35, 0.9716, 0.3365, 1.9E-44, 1.3E-19, 5.1E-6, 5.3E-20, 1.9E-50, and 2.3E-34); f (0.424, 0.8355, 0.9635, 5.8E-16, 0.2608, 0.34, 1.6E-10, 4.4E-8, 2.6E-13, 7.2E-29, 1.1E-62, and 3.5E-35). Source data are provided in this paper or the Mendeley database (https://data.mendeley.com/datasets/n9txt6y5cj/1). Similar results for (a–c) panels were obtained in two independent experiments.