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. 2023 Mar 31;13:5280. doi: 10.1038/s41598-023-32433-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Top canonical pathway analysis and the kinome tree analysis of the adipose tissue phospho-proteome of postpartum (PP) dairy cows supplemented with ALA 20 min after glucose infusion. Dairy cows were divided into two groups from 21 to 60 days PP; (i) CTL—encapsulated saturated fat, (ii) ALA—flaxseed supplement providing α-linolenic acid (ALA). n = 5 per treatment. (A) Top canonical pathways associated with ALA supplementation of dairy cows on the Y-axis and—log (P-value) on the X-axis. The size and color of each bubble represent the number of peptides in each pathway and the P value, respectively. (B) The predicted kinases of phospho-proteome in ALA-supplemented AT as represented in the kinome tree using the kinmap server. The identified kinases of the Phosphoproteome of dairy cows are represented as follows: AGC PKA/PKG/PKC-family kinases, CAMK calcium/calmodulin-dependent kinases, CK1 casein kinases, CMGC CDK/MAPK/GSK3/CLK-family kinases, STE sterile homologue kinases, TK tyrosine kinases, TKL tyrosine kinase-like kinases. The kinases are separated and denoted as red circles on the branches of the tree based on their category.