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. 2022 Jun 11;10(1):65–68. doi: 10.1016/j.gendis.2022.05.030

Supplementary Fig. 3.

Supplementary Fig. 3

The gene editing strategy of Sema4D knock out mice model. (A) Transwell assay of GN11 cells transfected with si Raf1in Mouse-Sema4D-WT-CM and Mouse-Sema4D-Ala515Val-CM treatment groups. (B) Scratch assay of GN11 cells transfected with si Raf1in Mouse-Sema4D-WT-CM and Mouse-Sema4D-Ala515Val-CM treatment groups. (C) The gRNA target sequence used to knock out Sema4D in mice models. (D) The strategy of the establishment of Sema4D knock-out mice model. Finally, the axon 5, 6, 7 were deleted in Sema4D in genome DNA. (E) Verification of Sema4D knock-out mice model establishment. Total 5591bp nucleotides were deleted.