Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

letter

. 2023 Mar 1;20(4):423–426. doi: 10.1038/s41423-023-00986-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — The in vivo distribution and migration pattern of tumor-specific memory CD8⁺ T cells during tumorigenesis. a Statistical summary of the proportions of CD45.1⁺CD44⁺ P14 cells (upper panel) and CD45.1⁺CD44⁺TCF1⁺TOX⁻ T_TSM cells (lower panel) in gated live lymphocytes from each compartment. BM bone marrow, nLNs nondraining lymph nodes, including axillary lymph nodes and submaxillary lymph nodes, TdLNs tumor-draining lymph nodes. n ≥ 4/group. b Flow cytometry analyses of TOX expression versus the indicated markers in CD45.1⁺CD44⁺TCF1⁺ donor P14 cells in TdLNs. c Experimental design of the parabiotic system. d Representative flow-cytometry plots of donor T_TSM-derived CD44 + P14 cells in the TME, TdLNs, and PBMCs of the donor (D) and host/recipient (R) tumor-bearing parabionts (right panel). Donor-derived CD44+ P14 cells in the spleens, TdLNs, and PBMCs of donor- and recipient-infected parabionts are listed in the left panel. Numbers are frequencies. e The ratio of donor-derived CD44+ P14 cells from each indicated compartment in the recipient relative to the donor is summarized. f Flow-cytometry analyses of GFP (indicating TCF-1 expression) versus indicated exhaustion and effector cell-associated marker expression in CD45.1⁺CD44⁺ donor P14 cells from different compartments. n = 3/group. *p < 0.05 versus control, n.s. stands for not significant, paired two-tailed Student’s t test (d). Data are representative of 2 independent experiments (mean ± SEM)