Fig. 5. Pdgfrβ-KO induced beige fat development is mediated by IL-33.

a Experimental schema: TMX-induced 12-month-old Sma-Control and Sma-Pdgfrβ-KO male mice were administered one dose of mouse IgG (1 µg/mouse) or α-IL-33 (1 µg/mouse) antibodies for 5 consecutive days and subsequently cold exposed for 2 or 7 days. b mRNA levels of IL-13 and IL-5 within iWAT depots from 2-day cold exposed mice described in (f) (n = 4 mice/group). c Representative H&E staining of dorsolumbar iWAT sections from 7-day cold exposed mice described in (a) (×10 magnification, scale bars 100 µm). d Representative Plin1 (blue) and Ucp1 (green) immunostaining from iWAT sections from 7-day cold exposed mice described in (a) (×20 magnification, scale bars 100 µm) (Images representative of 2 independent experiments). e Experimental schema: 2-month-old TMX-induced Sma-Control and Sma-Pdgfrβ^D849V were administered one dose of vehicle (0.1%BSA in 1xPBS) or recombinant murine IL-33 (12 µg/kg) for 5 consecutive days and subsequently cold exposed for 2 or 7 days. f mRNA levels of IL-13 and IL-5 within iWAT depots from 2-day cold exposed mice described in (e) (n = 4 mice/group). g Representative H&E staining of dorsolumbar iWAT sections from vehicle or IL-33 treated Pdgfrβ^D849V 7-day cold exposed mice as described in (e) (×10 magnification, scale bars 100 µm). h Representative of Plin1 (blue) and Ucp1 (green) immunostaining of dorsolumbar iWAT sections from Control and Pdgfrβ^D849V 7-day cold exposed mice (×20 magnification, scale bars 100 µm) (Images representative of 2 independent experiments). Data are presented as mean values ± SEM. Data were analyzed by two-tailed Student’s t-test or two-way ANOVA for multiple comparisons. Source data are provided within the Source data file.