Fig. 3.

PLZF deficiency protects mice from C. rodentium infection. A–E Six- to eight-week-old WT or PLZF^−/− mice were sacrificed, and LI LPLs were isolated for analysis (n = 3). A–D Representative flow cytometry plots for IL-22 (A), GM-CSF (B), IFN-γ (C), and IL-17A (D) produced by ILC3s gated on CD45.2⁺Lin⁻CD90.2⁺. E Frequencies of each cytokine in ILC3s in WT and PLZF^−/− mice. F–I Six- to eight-week-old WT or PLZF^−/− mice were infected with C. rodentium (n = 3–6). Samples were collected and analyzed at the indicated time points. F Bacterial load after C. rodentium infection on Day 6. G Representative image of H&E staining of colon sections from C. rodentium–infected mice at Day 12 p.i. Scale bar is 100 μm. H Relative mRNA expression of Reg3β, Reg3γ and IL-22 in colonic tissue homogenates of infected mice was analyzed by real-time RT‒PCR. I Representative flow cytometry plots for IL-22-expressing ILC3s in large intestine LPLs after C. rodentium infection on Day 6. J, K Frequencies of IL-22 in ILC3s and lymphocytes in the large intestine LPLs. A‒K Data are representative of at least two independent experiments. Data are represented as the mean ± SEM. Error bars show SEM. *P < 0.05; **P < 0.01; ***P < 0.01