Fig. 5.

PLZF regulates ILC3 function and gut immunity in a cell-intrinsic manner. A Experimental scheme for ILC3 adoptive transfer. ILC3s (Lin⁻CD45^midCD90^hi) sorted from the large intestine LPLs of WT and PLZF^−/− mice were transplanted into Rag2^−/−Il2rg^−/− recipients (1 × 10^{^5} ILC3s per recipient), and recipients were subjected to C. rodentium infection by gavage 8 weeks later (n = 3–4). B Representative flow cytometry plots showing ILC3 reconstitution in the small intestine and the large intestine. C Bacterial load after C. rodentium infection on Day 6. D Representative flow cytometry plots for IL-22-expressing ILC3s from the large intestine are shown from the recipients. E Frequencies of IL-22⁺ in ILC3s. F Relative mRNA expression of Reg3γ, Reg3β and IL-22 in colonic tissue homogenates of infected recipients on Day 6 p.i. was analyzed by real-time RT‒PCR. G Bone marrow from the CD45.2 PLZF^−/− or CD45.1.2 WT mice were mixed 1:1 and transplanted into lethally irradiated recipients (CD45.1⁺). IL-22 production by ILC3s from donor BM-derived ILC3s (CD45.2⁺ PLZF^−/− and CD45.1.2⁺ WT) was determined by flow cytometry after 8 weeks of transfer. A–G Data are representative of at least two independent experiments. Data are represented as the mean ± SEM. Error bars show SEM. *P < 0.05; **P < 0.01; ***P < 0.001