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. 2023 Mar 31;13:5237. doi: 10.1038/s41598-023-32378-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Illustration of data synthesis and data analysis. Two adaptive sequencing runs with (a) readfish, (b) UNCALLED, and (c) one control run were conducted on the synthetic metagenomic sample with 95% human DNA, 4.9% Zymo DNA, and 0.1% TB DNA. Adaptive sequencing can adaptively select or reject a sequencing read on each flowcell. (d) An Amplicon run was conducted on a selected TB gene region. All generated reads underwent benchmarking to compare different methods of enrichment of TB reads.