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. 2023 Mar 31;13:5237. doi: 10.1038/s41598-023-32378-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Read length distributions among different samples. The read cumulative percentage over different read lengths (bp) from different species at samples generated via adaptive sequencing of (a) a readfish run, (b) UNCALLED run, (c) Control run, and (d) Amplicon run. Compared with the control run, in which different species reads had similar read length distribution, the readfish and UNCALLED runs had a distinct pattern; the target species, TB, had a much longer read length, while the non-target reads, human and Zymo, had a limited read length (99% of human and Zymo reads < 500 bp in readfish and < 2900 bp in UNCALLED).