Fig. 8. Endfoot mitochondria in young and aging mice do not rely on extracellular Ca²⁺.

Representative mitochondrial Ca²⁺ event traces from young (a) or aging (c) mice before and after bath application of zero Ca²⁺ aCSF. ROIs were averaged across individual blood vessels to compare frequency (left), amplitude (middle), and duration (right) for mitochondrial Ca²⁺ events recorded in young (b) or aging (d) mice. For young mice, n = 52 ROIs and 18 blood vessels from 3 mice. For aging mice, n = 28 ROIs and 16 blood vessels from 5 mice. Error bars are S.E.M. All frequency and duration p values and the aging amplitude p value are based paired sample t-tests and the young amplitude p value is based on a Wilcoxon signed ranked test.