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. 2023 Mar 31;14:1800. doi: 10.1038/s41467-023-36808-2

Fig. 3. Anatomical and neurochemical characterization of DRN-LHb and DRN-BNST circuits.

Fig. 3

A Representative CtB 647 infection in BNST, females. Similar images were obtained in n = 3 mice B Representative CtB 555 infection, females. Similar images were obtained in n = 3 mice. C Representative CtB 647 infection in BNST, males. Similar images were obtained in n = 4 mice. D Representative CtB 555 infection in LHb, males. Similar images were obtained in n = 4 mice. E Representative CtB 647 labeling in DRN, females. Similar images were obtained in n = 3 mice. F Representative CtB 555 labeling in DRN, females. Similar images were obtained in n = 3 mice. G Representative CtB 647 infection in BNST, males. Similar images were obtained in n = 4 mice. H Representative CtB 555 infection, males. Similar images were obtained in n = 4 mice. I Representative 5HT labeling in DRN, females. Similar images were obtained in n = 3 mice. J Representative labeling in DRN for CtB 647, CtB 555, and 5HT, females. Similar images were obtained in n = 3 mice. K Representative 5HT labeling in DRN, males. Similar images were obtained in n = 4 mice. L Representative labeling in DRN for CtB 647, CtB 555, and 5HT, males. Similar images were obtained in n = 4 mice. M Percent of DRN-LHb neurons positive for 5HT N Percent of DRN-BNST neurons positive for 5HT. O Percent of CtB 555 neurons that co-express CtB 647 in DRN. P Percent of CtB 647 neurons that co-express CtB 555 in DRN. Q In-situ hybridization for 5HT2c, vGAT, and vGlut2 in BNST, females. Similar images were obtained in n = 3 mice. R In-situ hybridization for 5HT2c, vGAT, and vGlut2 in BNST, males. Similar images were obtained in n = 4 mice. S, In-situ hybridization for 5HT2c, vGAT, and vGlut2 in LHb, females. Similar images were obtained in n = 3 mice. T In-situ hybridization for 5HT2c, vGAT, and vGlut2 in LHb, males. Similar images were obtained in n = 4 mice. U Dorsal BNST 5HT2c neuron overlaps with vGAT or vGlut2. V Ventral BNST 5HT2c neuron overlaps with vGAT or vGlut2. W LHb 5HT2c neuron overlap with vGAT or vGlut2. n = 3 males, n = 4 females, 2 slices/mouse. No statistical comparisons were performed on this data as they are intended to be descriptive. All data are represented as mean ± SEM. Source data are provided as a Source Data file. Created with Biorender.com.