Fig. 7. DiD modulates the calcium responses of LHb5HT2c and BNST5HT2c neurons to affective stimuli.
A Surgical schematic and experimental timeline for LHb5HT2c and BNST5HT2c GCaMP fiber photometry experiments in DiD-exposed mice. B Average alcohol consumption in DiD (n = 12 females, n = 8 males). C Cumulative alcohol intake in DiD (n = 12 females, n = 8 males). D Average alcohol preference in DiD (n = 12 females, n = 8 males). E Peri-event plot of LHb5HT2c GCaMP activity during the first interaction with a novel social target, females (water n = 10 mice/1 trial, DiD n = 11 mice/1 trial). F Peak z-score of LHb5HT2c GCaMP signal for 0–1 s post interaction, females, Mann–Whitney U test (water n = 10 mice/1 trial, DiD n = 11 mice/1 trial). G Peri-event plot of LHb5HT2c GCaMP activity during the first interaction with a novel social target, males (water n = 7 mice/1 trial, DiD n = 8 mice/1 trial). H Average z-score of LHb5HT2c GCaMP signal for 0–5 s post interaction, males (water n = 7 mice/1 trial, DiD n = 8 mice/1 trial). I Peri-event plot of BNST5HT2c GCaMP activity during the first interaction with a novel social target, females (water n = 10 mice/1 trial, DiD n = 12 mice/1 trial). J Average z-score of BNST5HT2c GCaMP signal for 0–5 s post interaction, females (water n = 10 mice/1 trial, DiD n = 12 mice/1 trial). K Peri-event plot of BNST5HT2c GCaMP activity during the first interaction with a novel social target, males (water n = 7 mice/1 trial, DiD n = 8 mice/1 trial). L Average z-score of BNST5HT2c GCaMP signal for 0–5 s post interaction, males (water n = 7 mice/1 trial, DiD n = 8 mice/1 trial). M Peri-event plot of LHb5HT2c GCaMP activity during the acoustic startle test, females (water n = 10 mice/10 trials, DiD n = 11 mice/10 trials). N Average z-score of LHb5HT2c GCaMP signal for 0–5 s post acoustic startle stimulus, females (water n = 10 mice/10 trials, DiD n = 11 mice/10 trials). O Peri-event plot of LHb5HT2c GCaMP activity during the acoustic startle test, males (water n = 7 mice/10 trials, DiD n = 8 mice/10 trials). P Average z-score of LHb5HT2c GCaMP signal for 0–5 s post acoustic startle stimulus, males, p = 0.0008 (water n = 7 mice/10 trials, DiD n = 8 mice/10 trials). Q Peri-event plot of BNST5HT2c GCaMP activity during the acoustic startle test, females (water n = 10 mice/10 trials, DiD n = 12 mice/10 trials). R Average z-score of BNST5HT2c GCaMP signal for 0–5 s post acoustic startle stimulus, females (water n = 10 mice/10 trials, DiD n = 12 mice/10 trials). S Peri-event plot of BNST5HT2c GCaMP activity during the acoustic startle test, males (water n = 7 mice/10 trials, DiD n = 8 mice/10 trials). T Average z-score of BNST5HT2c GCaMP signal for 0–5 s post acoustic startle stimulus, males, p = 0.0157 (water n = 7 mice/10 trials, DiD n = 8 mice/10 trials). Unless otherwise stated, statistical comparisons were performed using two-way Student’s unpaired t tests. All data represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.0001. Source data are provided as a Source Data file. Created with Biorender.com.