Table 1.

KAT II inhibitor, PF-04859989, alters sleep onset and transitions between vigilance states.

		Phase	Econ vehicle	ECon PF-04859989	EKyn vehicle	EKyn PF-04859989	Three-way ANOVA
PF-04859989 at ZT 0	NREM onset (min)	Light	19 ± 3	19 ± 3	19 ± 3	17 ± 2
		Dark	31 ± 11	26 ± 3	38 ± 14	21 ± 4
	REM onset (min)	Light	56 ± 5	56 ± 6	56 ± 4	54 ± 5	# Treatment
		Dark	93 ± 29	49 ± 7	97 ± 31	59 ± 14
	Wake to NREM (#)	Light	118 ± 6	116 ± 5	109 ± 5	99 ± 6
		Dark	77 ±5	77 ± 8	85 ± 7	78 ± 8
	NREM to REM (#)	Light	45 ± 2	49 ± 2	43 ± 3	47 ± 3
		Dark	23 ± 2	24 ± 2	24 ± 2	23 ± 3
	REM to wake (#)	Light	29 ± 1	30 ± 1	30 ± 2	30 ± 2
		Dark	17 ± 2	18 ± 2	17 ± 2	15 ± 2
	NREM to wake (#)	Light	89 ± 6	86 ± 5	79 ± 5	69 ± 5
		Dark	60 ± 5	58 ± 8	67 ± 7	62 ± 8
	REM to NREM (#)	Light	16 ± 2	19 ± 2	13 ± 2	16 ± 2	# Treatment
		Dark	6 ± 1	6 ± 1	6 ± 1	8 ± 2
PF-04859989 at ZT 12	NREM onset (min)	Dark	33 ± 7	33 ± 10	32 ± 4	30 ± 5
		Light	12 ± 4	9 ± 3	11 ± 5	2 ± 0
	REM Onset (min)	Dark	84 ± 22	108 ± 23	90 ± 19	120 ± 26
		Light	45 ± 16	36 ± 7	41 ± 13	19 ± 2
	Wake to NREM (#)	Dark	77 ± 9	83 ± 8	81 ± 5	87 ± 5	## Treatment
		Light	113 ± 13	125 ± 13	112 ± 9	120 ± 10
	NREM to REM (#)	Dark	21 ± 3	22 ± 3	17 ± 3	18 ± 3
		Light	40 ± 3	40 ± 5	32 ± 3	35 ± 3
	REM to wake (#)	Dark	17 ± 2	17 ± 2	15 ± 2	16 ± 3
		Light	29 ± 3	31 ± 3	27 ± 3	29 ± 2
	NREM to wake (#)	Dark	60 ± 7	66 ± 7	67 ± 5	70 ± 5	## Treatment
		Light	84 ± 11	94 ± 11*	85 ± 8	91 ± 10
	REM to NREM (#)	Dark	4 ± 2	5 ± 2	2 ± 1	2 ± 1
		Light	11 ± 2	10 ± 2	5 ± 1	7 ± 1

Data are mean ± SEM. Three-way RM ANOVA: ^#P < 0.05, ^##P < 0.01. Two-way RM ANOVA with Fisher’s LSD post hoc test: *P < 0.05. N = 8–16 per group.